Load all required libraries.
library(tidyverse)
## -- Attaching packages --------------------------------------- tidyverse 1.3.1 --
## v ggplot2 3.3.5 v purrr 0.3.4
## v tibble 3.1.3 v dplyr 1.0.7
## v tidyr 1.1.3 v stringr 1.4.0
## v readr 2.0.0 v forcats 0.5.1
## -- Conflicts ------------------------------------------ tidyverse_conflicts() --
## x dplyr::filter() masks stats::filter()
## x dplyr::lag() masks stats::lag()
library(plotly)
##
## Attaching package: 'plotly'
## The following object is masked from 'package:ggplot2':
##
## last_plot
## The following object is masked from 'package:stats':
##
## filter
## The following object is masked from 'package:graphics':
##
## layout
library(broom)
Read in raw data from RDS.
raw_data <- readRDS("./year2.RDS")
Make a few small modifications to names and data for visualizations.
final_data <- raw_data %>% mutate(log_copy_per_L = log10(mean_copy_num_L)) %>%
rename(Facility = wrf) %>%
mutate(Facility = recode(Facility,
"NO" = "WRF A",
"MI" = "WRF B",
"CC" = "WRF C"))
Seperate the data by gene target to ease layering in the final plot
#make three data layers
only_positives <<- subset(final_data, (!is.na(final_data$Facility)))
only_n1 <- subset(only_positives, target == "N1")
only_n2 <- subset(only_positives, target == "N2")
only_background <<-final_data %>%
select(c(date, cases_cum_clarke, new_cases_clarke, X7_day_ave_clarke)) %>%
group_by(date) %>% summarise_if(is.numeric, mean)
#specify fun colors
background_color <- "#7570B3"
seven_day_ave_color <- "#E6AB02"
marker_colors <- c("N1" = '#1B9E77',"N2" ='#D95F02')
#remove facilty C for now
#only_n1 <- only_n1[!(only_n1$Facility == "WRF C"),]
#only_n2 <- only_n2[!(only_n2$Facility == "WRF C"),]
only_n1 <- only_n1[!(only_n1$Facility == "WRF A" & only_n1$date == "2020-11-02"), ]
only_n2 <- only_n2[!(only_n2$Facility == "WRF A" & only_n2$date == "2020-11-02"), ]
Build the main plot
#first layer is the background epidemic curve
p1 <- only_background %>%
plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~new_cases_clarke,
type = "bar",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Daily Cases: ', new_cases_clarke),
alpha = 0.5,
name = "Daily Reported Cases",
color = background_color,
colors = background_color,
showlegend = FALSE) %>%
layout(yaxis = list(title = "Clarke County Daily Cases", showline=TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#renders the main plot layer two as seven day moving average
p1 <- p1 %>% plotly::add_trace(x = ~date, y = ~X7_day_ave_clarke,
type = "scatter",
mode = "lines",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Seven-Day Moving Average: ', X7_day_ave_clarke),
name = "Seven Day Moving Average Athens",
line = list(color = seven_day_ave_color),
showlegend = FALSE)
#renders the main plot layer three as positive target hits
p2 <- plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n1,
symbol = ~Facility,
marker = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n2,
symbol = ~Facility,
marker = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(yaxis = list(title = "SARS CoV-2 Copies/L",
showline = TRUE,
type = "log",
dtick = 1,
automargin = TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#adds the limit of detection dashed line
p2 <- p2 %>% plotly::add_segments(x = as.Date("2021-06-30"),
xend = ~max(date + 10),
y = 3571.429, yend = 3571.429,
opacity = 0.35,
line = list(color = "black", dash = "dash")) %>%
layout(annotations = list(x = as.Date("2021-06-30"), y = 3.8, xref = "x", yref = "y",
text = "Limit of Detection", showarrow = FALSE))
p1
p2
Combine the two main plot pieces as a subplot
#seperate n1 and n2 frames by site
#n1
wrf_a_only_n1 <- subset(only_n1, Facility == "WRF A")
wrf_b_only_n1 <- subset(only_n1, Facility == "WRF B")
wrf_c_only_n1 <- subset(only_n1, Facility == "WRF C")
#n2
wrf_a_only_n2 <- subset(only_n2, Facility == "WRF A")
wrf_b_only_n2 <- subset(only_n2, Facility == "WRF B")
wrf_c_only_n2 <- subset(only_n2, Facility == "WRF C")
#rejoin the old data frames then seperate in to averages for each plant.
wrfa_both <- full_join(wrf_a_only_n1, wrf_a_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
wrfb_both <- full_join(wrf_b_only_n1, wrf_b_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
wrfc_both <- full_join(wrf_c_only_n1, wrf_c_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
#get max date
maxdate <- max(wrfa_both$date)
mindate <- min(wrfa_both$date)
Build loess smoothing figures figures
This makes the individual plots
#**************************************WRF A PLOT**********************************************
#add trendlines
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_botha <- ggplot(wrfa_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_botha<<-..y..), method = "loess", color = '#1B9E77',
span = 0.3, n = 92)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_botha
## `geom_smooth()` using formula 'y ~ x'
fit_botha
## [1] 11.59181 11.60102 11.61522 11.63378 11.65603 11.68132 11.70900 11.74233
## [9] 11.78359 11.83070 11.88155 11.93403 11.98605 12.04822 12.10724 12.13834
## [17] 12.14931 12.15102 12.15432 12.17006 12.22232 12.27929 12.31056 12.33611
## [25] 12.35857 12.38058 12.40479 12.43322 12.46490 12.50313 12.54896 12.59710
## [33] 12.64225 12.67911 12.71710 12.75044 12.76499 12.76903 12.76812 12.76781
## [41] 12.77366 12.77259 12.77382 12.79093 12.81219 12.83863 12.87128 12.91117
## [49] 12.97837 13.04778 13.10338 13.16726 13.22984 13.28153 13.31274 13.31461
## [57] 13.30827 13.29696 13.26695 13.22807 13.19019 13.16314 13.15459 13.14813
## [65] 13.13395 13.11929 13.10355 13.08614 13.06645 13.04390 13.01027 12.98728
## [73] 12.96824 12.95047 12.93132 12.90811 12.88865 12.86432 12.82399 12.77683
## [81] 12.72588 12.67418 12.62479 12.57633 12.52673 12.47584 12.42285 12.36878
## [89] 12.31462 12.26141 12.20953 12.15812
#assign fits to a vector
both_trenda <- fit_botha
#extract y min and max for each
limits_botha <- ggplot_build(extract_botha)$data
## `geom_smooth()` using formula 'y ~ x'
limits_botha <- as.data.frame(limits_botha)
both_ymina <- limits_botha$ymin
both_ymaxa <- limits_botha$ymax
#reassign dataframes (just to be safe)
work_botha <- wrfa_both
#fill in missing dates to smooth fits
work_botha <- work_botha %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_botha <- work_botha$date
#create a new smooth dataframe to layer
smooth_frame_botha <- data.frame(date_vec_botha, both_trenda, both_ymina, both_ymaxa)
#WRF A
#plot smooth frames
p_wrf_a <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_botha, y = ~both_trenda,
data = smooth_frame_botha,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_botha,
'</br> Median Log Copies: ', round(both_trenda, digits = 2)),
line = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_botha, ymin = ~both_ymina, ymax = ~both_ymaxa,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_botha, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxa, digits = 2),
'</br> Min Log Copies: ', round(both_ymina, digits = 2)),
name = "",
fillcolor = '#1B9E77',
line = list(color = '#1B9E77')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF A") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfa_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#1B9E77', size = 6, opacity = 0.65))
p_wrf_a
save(p_wrf_a, file = "./site_objects/wrf_a_year2.rda")
#**************************************WRF B PLOT**********************************************
#add trendlines
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_bothb <- ggplot(wrfb_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_bothb<<-..y..), method = "loess", color = '#D95F02',
span = 0.3, n = 92)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothb
## `geom_smooth()` using formula 'y ~ x'
fit_bothb
## [1] 10.64492 10.77883 10.90409 11.02118 11.13057 11.23274 11.32817 11.41387
## [9] 11.48801 11.55277 11.61034 11.66292 11.71270 11.74082 11.76780 11.81302
## [17] 11.85682 11.90193 11.95108 12.00699 12.07181 12.14275 12.22802 12.33082
## [25] 12.43899 12.54034 12.62272 12.68259 12.73405 12.78364 12.82741 12.86396
## [33] 12.89185 12.90967 12.90122 12.88639 12.87671 12.85411 12.82698 12.80374
## [41] 12.79278 12.79792 12.80704 12.81738 12.83476 12.85659 12.88027 12.90322
## [49] 12.92014 12.93720 12.96670 13.00574 13.04615 13.07978 13.09848 13.09527
## [57] 13.08647 13.07388 13.04680 13.01323 12.98121 12.95875 12.95089 12.94545
## [65] 12.93943 12.93926 12.94132 12.94195 12.93753 12.92440 12.89471 12.86079
## [73] 12.81370 12.76491 12.72590 12.70814 12.71486 12.73187 12.76749 12.82831
## [81] 12.89704 12.95638 12.98906 12.99427 12.99048 12.98162 12.96382 12.93710
## [89] 12.90145 12.85687 12.80333 12.74098
#assign fits to a vector
both_trendb <- fit_bothb
#extract y min and max for each
limits_bothb <- ggplot_build(extract_bothb)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothb <- as.data.frame(limits_bothb)
both_yminb <- limits_bothb$ymin
both_ymaxb <- limits_bothb$ymax
#reassign dataframes (just to be safe)
work_bothb <- wrfb_both
#fill in missing dates to smooth fits
work_bothb <- work_bothb %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothb <- work_bothb$date
#create a new smooth dataframe to layer
smooth_frame_bothb <- data.frame(date_vec_bothb, both_trendb, both_yminb, both_ymaxb)
#WRF B
#plot smooth frames
p_wrf_b <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_bothb, y = ~both_trendb,
data = smooth_frame_bothb,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothb,
'</br> Median Log Copies: ', round(both_trendb, digits = 2)),
line = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothb, ymin = ~both_yminb, ymax = ~both_ymaxb,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothb, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxb, digits = 2),
'</br> Min Log Copies: ', round(both_yminb, digits = 2)),
name = "",
fillcolor = '#D95F02',
line = list(color = '#D95F02')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF B") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfb_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#D95F02', size = 6, opacity = 0.65))
p_wrf_b
save(p_wrf_b, file = "./site_objects/wrf_b_year2.rda")
#**************************************WRF C PLOT********************************************** #add trendlines #extract data from geom_smooth # *********************************span 0.6*********************************** #*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_bothc <- ggplot(wrfc_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_bothc<<-..y..), method = "loess", color = '#E7298A',
span = 0.3, n = 92)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothc
## `geom_smooth()` using formula 'y ~ x'
fit_bothc
## [1] 10.55804 10.71141 10.84769 10.96927 11.07852 11.17783 11.26957 11.34214
## [9] 11.38777 11.41459 11.43075 11.44438 11.46361 11.47963 11.50291 11.58506
## [17] 11.72779 11.89331 12.04384 12.14158 12.20475 12.24634 12.22231 12.14136
## [25] 12.03708 11.94303 11.89280 11.88409 11.88776 11.90872 11.95135 12.00818
## [33] 12.07172 12.13449 12.21499 12.29714 12.37035 12.45435 12.53529 12.59938
## [41] 12.63278 12.62953 12.61309 12.56939 12.48354 12.38457 12.30152 12.26342
## [49] 12.27850 12.31156 12.37091 12.47129 12.58636 12.68980 12.75526 12.78066
## [57] 12.79416 12.79186 12.76596 12.72956 12.69577 12.67770 12.67040 12.66599
## [65] 12.67210 12.68680 12.70453 12.71971 12.72677 12.72013 12.70079 12.67086
## [73] 12.62815 12.57912 12.53025 12.48801 12.44997 12.41487 12.38664 12.36316
## [81] 12.34359 12.32707 12.31276 12.30245 12.29402 12.28527 12.27845 12.27355
## [89] 12.27056 12.26948 12.27026 12.27276
#assign fits to a vector
both_trendc <- fit_bothc
#extract y min and max for each
limits_bothc <- ggplot_build(extract_bothc)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothc <- as.data.frame(limits_bothc)
both_yminc <- limits_bothc$ymin
both_ymaxc <- limits_bothc$ymax
#reassign dataframes (just to be safe)
work_bothc <- wrfc_both
#fill in missing dates to smooth fits
work_bothc <- work_bothc %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothc <- work_bothc$date
#create a new smooth dataframe to layer
smooth_frame_bothc <- data.frame(date_vec_bothc, both_trendc, both_yminc, both_ymaxc)
#WRF C
#plot smooth frames
p_wrf_c <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_bothc, y = ~both_trendc,
data = smooth_frame_bothc,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothc,
'</br> Median Log Copies: ', round(both_trendc, digits = 2)),
line = list(color = '#E7298A', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothc, ymin = ~both_yminc, ymax = ~both_ymaxc,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothc, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxc, digits = 2),
'</br> Min Log Copies: ', round(both_yminc, digits = 2)),
name = "",
fillcolor = '#E7298A',
line = list(color = '#E7298A')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF C") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfc_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#E7298A', size = 6, opacity = 0.65))
p_wrf_c
save(p_wrf_c, file = "./site_objects/wrf_c_year2.rda")
keeping in case
#save(wrfa_both, file = "./plotly_objs/wrfa_both.rda")
#save(wrfb_both, file = "./plotly_objs/wrfb_both.rda")
#save(wrfc_both, file = "./plotly_objs/wrfc_both.rda")
#save(date_vec_botha, file = "./plotly_objs/date_vec_botha.rda")
#save(date_vec_bothb, file = "./plotly_objs/date_vec_bothb.rda")
#save(date_vec_bothc, file = "./plotly_objs/date_vec_bothc.rda")
#save(both_ymina, file = "./plotly_objs/both_ymina.rda")
#save(both_ymaxa, file = "./plotly_objs/both_ymaxa.rda")
#save(both_yminb, file = "./plotly_objs/both_yminb.rda")
#save(both_ymaxb, file = "./plotly_objs/both_ymaxb.rda")
#save(both_yminc, file = "./plotly_objs/both_yminc.rda")
#save(both_ymaxc, file = "./plotly_objs/both_ymaxc.rda")